Protocols for C-Brick DNA Standard Assembly Using Cpf1
Title:
Protocols for C-Brick DNA Standard Assembly Using Cpf1
Author:
Li, Shi-Yuan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (723 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
CRISPR-associated protein Cpf1 can be guided by a specially designed CRISPR RNA (crRNA) to cleave double-stranded DNA at desired sites, generating sticky ends. Based on this characteristic, a DNA assembly standard (C-Brick) was established, and a protocol detailing its use is described here.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55775