Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
Title:
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
Author:
Maliszewska-Olejniczak, Kamila
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (318 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61399