3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Title:
3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Author:
Marasca, Federica
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (685 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60712