A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Title:
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Author:
Marayati, Bahjat F.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (475 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
We present a simple suppressor screen protocol in fission yeast. This method is efficient, mutagen-free, and selective for mutations that often occur at a single genomic locus. The protocol is suitable for isolating suppressors that alleviate growth defects in liquid culture that are caused by a mutation or a drug.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59133