Front Cover -- Landmark Papers in Clinical Chemistry -- Copyright Page -- Contents -- Foreword -- Introduction -- Acknowledgments -- Claude Bernard -- Section I: Immunoassay Technology -- RIA (Radioimmunoassay) -- 1.Immunoassay of endogenous plasma insulin in man. Commentary -- Landmark Paper -- FPIA (Fluorescence Polarization Immunoassay) -- 2. Quantification of the antigen-antibody reaction by the polarization of fluorescence. Commentary -- Landmark Paper -- CPB (Competitive Protein Binding) -- 3. Determination of thyroxine utilizing the property of protein-binding. Commentary -- Landmark Paper -- ELISA (Enzyme-Linked Immunosorbent Assay) -- 4. Enzyme-linked immunosorbent assay II. Commentary -- Landmark Paper -- EMIT™ (Enzyme Immunoassay Technique) -- 5. "Homogeneous" enzyme immunoassay. A new immunochemical technique. Commentary -- Landmark Paper -- Section II: Therapeutic Drug Monitoring (TDM) -- Bromide -- 6. Rational bromide treatment. New methods for its control. Commentary -- Landmark Paper -- Sulfanilamide -- 7. A new coupling component for sulfanilamide determination. Commentary -- Landmark Paper -- Quinacrine -- 8. The estimation of Atabrine in biological fluids and tissues. Commentary -- Landmark Paper -- Digoxin -- 9. Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay. Commentary -- Landmark Paper -- Theophylline -- 10. Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography. Commentary -- Landmark Paper -- Section III: Enzymology -- ALP (Alkaline Phosphatase, EC 3.1.3.1) -- 11. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. Commentary -- Landmark Paper -- AST (Aspartate Aminotransferase, EC 2.6.1.1) -- 12. Transaminase activity in human blood. Commentary -- Landmark Paper.

CK (Creatine Kinase, EC 2.7.3.2) -- 13. Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography. Commentary -- Landmark Paper -- Section IV: Specific Analytes -- Colorimetric Analysis with the Duboscq -- 14. A system of blood analysis. Commentary -- Landmark Paper -- Blood Gases by Manometer -- 15. The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I. Commentary -- Landmark Paper -- Bilirubin With the Filter Photometer -- 16. The determination of bilirubin with the photoelectric colorimeter. Commentary -- Landmark Paper -- Proteins by the Biuret Reaction -- 17. Determination of serum proteins by means of the biuret reaction. Commentary -- Landmark Paper -- Methods for Pediatric and Neonatal Samples -- 18. Routine use of ultramicro methods in the clinical laboratory. Commentary -- Landmark Paper -- Enzymatic Glucose -- 19. Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. Commentary -- Landmark Paper -- HCG (Human Chorionic Gonadotropin) by Immunoassay -- 20. An immunological pregnancy test. Commentary -- Landmark Paper -- Hemoglobin Alc -- 21. An abnormal hemoglobin in red cells of diabetics. Commentary -- Landmark Paper -- LDL (Low Density Lipoprotein) Cholesterol by Calculation -- 22. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Commentary -- Landmark Paper -- Section V: Instrumentation and Techniques -- Paper Electrophoresis -- 23. A microelectrophoretic and microionophoretic technique. Commentary -- Landmark Paper -- Automated Analyzer -- 24. An automated method for colorimetric analysis. Commentary -- Landmark Paper -- Enzymatic Glucose, Dry Reagents on Pads -- 25. Simple specific test for urine glucose. Commentary.

Landmark Paper -- Blood Gas Electrodes -- 26. Electrodes for blood pO2 and pCO2 determination. Commentary -- Landmark Paper -- Specific Ion Electrodes -- 27. Single sample analysis with the sodium electrode. Commentary -- Landmark Paper -- Centrifugal Analyzer -- 28. Analytical techniques for cell fractions XII. A multiple-cuvet rotor for a new microanalytical system. Commentary -- Landmark Paper -- Immunoassays on Membranes -- 29. Immunocapillarymigration-A new method for immunochemical quantitation. Commentary -- Landmark Paper -- Western Blots -- 30. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Commentary -- Landmark Paper -- Capillary Electrophoresis (CE) -- 31. Zone electrophoresis in open-tubular glass capillaries. Commentary -- Landmark Paper -- Section VI: Chemometrics -- Quality Control Charts -- 32. The use of control charts in the clinical laboratory. Commentary -- Landmark Paper -- Interferences in Analytical Accuracy -- 33. Chemical and diagnostic specificity of laboratory tests. Commentary -- Landmark Paper -- Predictive Value Theory -- 34. Predictive value of a single diagnostic test in unselected populations. Commentary -- Landmark Paper -- Standards and Calibrators -- 35. What is a standard? Commentary -- Landmark Paper -- Test Kits -- 36. Performance of "kits" used for clinical chemical analysis of cholesterol. Commentary -- Landmark Paper -- Section VII: Molecular Diagnostics -- Biochemical Genetics -- 37. Sickle cell anemia, a molecular disease. Commentary -- Landmark Paper -- Nucleic Acid Probes -- 38. Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization. Commentary -- Landmark Paper -- Microarrays.

39. Oligonucleotide hybridisations on glass supports: a novel linker for oligonucleotide synthesis and hybridisation properties of aoligonucleotides synthesised in situ. Commentary -- Landmark Paper -- Overview of the Publication Source of the Original Articles -- Author Index -- Subject Index.