Contents -- Preface 1 Hans Dieter Klenk -- Preface 2 Yu-Mei Wen -- Section 1. Bacteria -- Introduction and Comments: Studies on Bacteria by Molecular and Experimental Approaches Yu-Mei Wen -- 1.1. Cloning, Expression and Immunological Evaluation of a Novel Protein Belonging to the OmpA Family in Leptospira interrogans Wei Lin, Hong-Liang Yang, Yang Yang, Bao-Yu Hu, Li-Zhi Tan and Xiao-Kui Guo -- 1. Introduction -- 2. Results -- 2.1. Bioinformatics analysis -- 2.2. Cloning and expression of the recombinant LA0301 in E. coli -- 2.3. The titer of serum anti-LA0301 and immunoreactivity of the recombinant LA0301 -- 2.4. The antibody of LA0301 was detected in anti-L. interrogans rabbit sera -- 2.5. Conservatism analysis of LA0301 in 15 different L. interrogans strains -- 3. Discussion -- References -- 1.2. Surveillance of Leptospirosis and the Murine Reservoirs in China, 2005 Xiu-Gao Jiang, Xiang-Yan Zhang, Wen-Jun Zhong, Yi-Xin Nie, Wen-Wu Yin, Xiu-Wen Li and Xiao-Kui Guo -- 1. Introduction -- 2. Results -- 3. Discussion -- Acknowledgments -- References -- 1.3. Cloning and Expression of the Histidinol Dehydrogenase Gene from Mycobacterium tuberculosis H37Rv and Properties of the Recombinant Histidinol Dehydrogenase Rui-Liang Jin, Rui-Qing Liu, Hong-Jun Wang, Jian Cao, Sheng-Feng Xu, Li-Juan Chunyu, Yun-Min Xu and Hong-Hai Wang -- 1. Introduction -- 2. Results -- 2.1. Sequence analysis of the PCR product -- 2.2. Expression and purification of MtHDH -- 2.3. Enzymatic properties of MtHDH -- 2.3.1. Enzyme activity and specific activity of MtHDH -- 2.3.2. Isoelectric point and optimal pH for enzyme activity -- 2.3.3. Effect of temperature on the enzyme activity and heat stability of MtHDH -- 2.3.4. Effect of different ions on the enzyme activity of the recombinant MtHDH -- 2.3.5. The Michaelis constant of MtHDH -- 2.4. HPLC and MS analysis.

2.5. Preliminary assay of the MtHDH secondary structure -- 3. Discussion -- References -- 1.4. Cloning and Sequence Analysis of the fbps Gene of Streptococcus suis Type 2 Li-Yun Sun, Hong-Jie Fan and Cheng-Ping Lu -- 1. Introduction -- 2. Results -- 2.1. PCR amplification -- 2.2. Construction of a recombinant plasmid -- 2.3. Analysis of the fbps gene sequence -- 2.4. Deduced FBPS protein sequence and analysis of the protein structure -- 3. Discussion -- References -- 1.5. Effects of EnvZ Histidine Kinase Inhibitors on the Virulence of Shigella flexneri Ming-Liang Chen, Qi Wang, Sheng-Fu Dong, Zhi-Qiang Qin, Jian Zhang, Cong Han, Xia Cai, Xu Shen and Di Qu -- 1. Introduction -- 2. Results -- 2.1. Expression and purification of recombinant SF-EnvZc -- 2.2. IC50 values of candidate SF-EnvZc inhibitors -- 2.3. Binding affinities of candidate compounds to SF-EnvZc -- 2.4. Mouse Sereny test -- 3. Discussion -- References -- 1.6. Detection of Virulence and Genes for Viable but Nonculturable (VBNC) Campylobacter jejuni Jie-Lin Yang, Tong-Hai Dou, Ming Gu and Zhong-Liang Wu -- 1. Introduction -- 2. Results -- 2.1. Bacterial counts -- 2.2. Cells for detection of enterotoxin -- 2.3. RT-PCR detection of C. jejuni intestinal toxin gene expression -- 3. Discussion -- References -- Section 2. Viruses -- Introduction and Comments: Trends on Virus Research in China Yu-Mei Wen -- 2.1. Transcriptomic and Proteomic Studies on HBsAg-Positive Transgenic Mouse Livers Suggest Alterations in Carbohydrate, Lipid and Amino Acid Metabolism Jun Ren, Chao Zhao, Cai-Yun Fang, Zhang-Mei Ma, Peng-Yuan Yang and Yu-Mei Wen -- 1. Introduction -- 2. Results -- 2.1. Microarray analysis and real-time quantitative RT-PCR assay of liver tissue of the transgenic mouse -- 2.2. Proteomic analysis of liver tissue of the transgenic mouse -- 3. Discussion -- References.

2.2. Large-Scale Polymorphism Analysis of Hepatitis B Virus Envelope Protein using Bioinformatics Zhe Chen, Yue Zhu, Yi-Xue Li and Yu-Mei Wen -- 1. Introduction -- 2. Results -- 2.1. Comparison between the conservation of the S region and of the pre-S region -- 2.2. Conservation and substitution patterns of amino acids in the pre-S (pre-S1 and pre-S2) regions -- 2.3. Bioinformatics analysis of known biological domains in the pre-S region -- 2.4. Topology of the S region -- 2.5. Conservation and substitution patterns of amino acids in the S region -- 2.6. Bioinformatics analysis of known biological domains in the S region -- 2.7. Bioinformatics analysis of N-glycosylations in envelopment proteins -- 3. Discussion -- References -- 2.3. Studies on the Inactivation of Hepadnavirus in Blood Components by Using a Duck Hepatitis B Virus-Infected Animal Model Wen-Yi Wang, Yu-Wen Huang, Ping Lu, Jian-Er Long, Qin Mo, Bing Ling, Xiao-Ling Han, Sheng-Fu Dong, Di Qu and Kai-Cheng Qian -- 1. Introduction -- 2. Results -- 2.1. DHBV-infected model and determination of a 50% infective dose of DHBV -- 2.2. Inactivation of DHBV infectivity by the MB photodynamic method -- 3. Discussion -- References -- 2.4. Sequence Analysis of the SH Gene and Its Flanking Region of a Mumps Virus Strain (SP Strain) Shao-Hui Ma, Rong-Song Zhang, Long-Ding Liu, Jing-Jing Wang, Li-Chun Wang, Wen-Jun Yang, Yan Liang, Zi-Jiang Yang and Qi-Han Li -- 1. Introduction -- 2. Results -- 2.1. Amplification of the SH gene and its flanking region of the mumps virus -- 2.2. Phylogenetic analysis -- 2.3. Analysis of the amino acid sequence of the SH gene -- 3. Discussion -- References.

2.5. HIV-Related Knowledge, Attitudes and Behaviors Among Policewomen and Female Prisoners from a Behavior Correction Center Wei Jiao, Ying-Jie Zheng, Xue-Cai Wang, Ai-Qin Xu, Jian-Ping Gu, Jian-Fu Zhu, Yi-Han Lu, Fa-Di Wang and Qing-Wu Jiang -- 1. Introduction -- 2. Results -- 2.1. Basic information on interviewees -- 2.2. Knowledge about AIDS among the policewomen and female prisoners -- 2.2.1. Resources for gaining knowledge -- 2.2.2. Attitude toward AIDS -- 2.2.3. High-risk behaviors leading to AIDS -- 2.3. Need for health knowledge and intervention -- 2.4. Effect of social concern -- 3. Discussion -- References -- Section 3. Laboratory Medicine -- Introduction and Comments: Advances and Trends in Diagnostic Microbiology in China Yi-Wei Tang -- 3.1. Detection of SARS-Associated Coronavirus Infection by Reverse Transcriptase-Polymerase Chain Reaction, Indirect Immunofluorescence Assay and Enzyme-Linked Immunosorbent Assay Zhi-Tong Zhou, Wei-Lun Jiang, Zheng-Hong Yuan, Fang Shen, Di Qu, Shi-Min Gu, Long-Ting Fu and Ren-Fang Zhang -- 1. Introduction -- 2. Results -- 2.1. RT-PCR assay for SARS-CoV RNA -- 2.2. IFA assay for SARS-CoV IgG antibody -- 2.3. ELISA assay for SARS-CoV antibody -- 2.4. Comparison of three different detection methods -- 3. Discussion -- References -- 3.2. Bacteriological and Molecular Identification of a Sporadic Case of Brucellosis in Shanghai Cheng-Yan Meng, Fei-Yi Ruan, Jian-Qiang Lei, Jia-Lin Jin and Wen-Hong Zhang -- 1. Introduction -- 2. Results -- 2.1. Microbiological tests -- 2.2. Results of BLAST -- 2.3. VNTR locus analysis -- 3. Discussion -- References -- 3.3. A PCR-Capillary Electrophoresis Method Detects and Differentiates Biovars of Ureaplasma urealyticum Shi-Hang Zhou and Zhuo-Ran Zhang -- 1. Introduction -- 2. Results -- 2.1. PCR-CE of reference strains of U. urealyticum -- 2.2. Analysis of sensitivity.

2.3. Analysis of specificity -- 2.4. Detection of N. gonorrhoeae, C. trachomatis, U. urealyticum biovar 1 and biovar 2 in men -- 3. Discussion -- References -- 3.4. Implications of CD64 in Early Diagnosis of Neonatal Septic Cases Rong Zhang and Chao Chen -- 1. Introduction -- 2. Results -- 2.1. The expression of CD64 -- 2.2. The levels of CRP -- 2.3. The sensitivity and specificity of CD64 and CRP in the diagnosis of neonatal septicemia -- 2.4. Dynamic changes of CD64 during septicemia -- 3. Discussion -- References -- 3.5. Correlation Between the MICA*A5.1 Allele and Chlamydia trachomatis Infection Bing Mei, Kun Du, Zhi Huo, Fu-Yan Wang, Shu-Hui Ma and Ping Yu -- 1. Introduction -- 2. Results -- 2.1. MICA gel electrophoresis map -- 2.2. The MICA*A5.1 allele and Ct infection in the infertile and control groups -- 2.3. Correlation between Ct infection and the MICA allele in the infertile and control groups -- 3. Discussion -- References -- 3.6. Surveillance of Human Infections with Avian Influenza in Guangzhou During 1997-2006 Peng-Zhe Qin, Yu-Lin Wang, Biao Di, Xiu-Zhen Zhou, Yang Liu, Yan-Shan Cai, Ji-Chuan Shen, Yu-Fei Liu, En-Jie Lu, Duan-Hua Zhou and Ming Wang -- 1. Introduction -- 2. Results -- 2.1. Serosurveillance -- 2.2. Case study -- 2.2.1. Case 1: H9N2 case -- 2.2.2. Case 2: First H5N1 case -- 2.2.3. Case 3: H5N1 infection of a poultry slaughterer -- 2.2.4. Case 4: H5N1 infection with no contact with poultry -- 3. Discussion -- References -- 3.7. Clinical Significance of Human Cytomegalovirus pp65-Specific Antibodies for Verification of HCMV Primary Infection Qian Liu, Jing-Xian Chen and Ming-Li Wang -- 1. Introduction -- 2. Results -- 2.1. HCMV pp65 antibody measured by indirect ELISA -- 2.2. HCMV virus isolation -- 2.3. Calculation of the kappa value -- 2.4. Judgment of primary infection -- 3. Discussion -- References.

3.8. Analysis of Indeterminate HIV Western Blot Profiles Kai Gao, Yan Li, Cai-Yun Liang, Hui-Fang Xu and Zhi-Gang Han.