v. 1. Production and in vivo applications of gene transfer vectors. Preparation and quantification of pseudotyped retroviral vector / Hong Yu and Young Jik Kwon. Production of retroviral vectors for clinical use / Kenneth Cornetta, Lilith Reeves, and Scott Cross. Methods for the production of helper-dependent adenoviral vectors / Donna J. Palmer and Philip Ng. Methods for the production of first generation adenoviral vectors / Donna J. Palmer and Philip Ng. Large-scale production of recombinant adeno-associated viral vectors / Alejandro Negrete and Robert M. Kotin. Construction and production of recombinant herpes simplex virus vectors / William F. Goins ... [et al.] Plasmid-based gene transfer in mouse skeletal muscle by electroporation / Jonathan D. Schertzer and Goron S. Lynch. Chitosan nanoparticle-mediated gene transfer / Dongwon Lee and Shyam S. Mohapatra. PEG-PEI copolymers for oligonucleotide delivery to cells and tissues / Gordon J. Lutz, Shashank R. Sirsi, and Jason H. Williams. Non-viral gene delivery with cationic liposome-DNA complexes / Kai K. Ewert ... [et al.] Applications of lentiviral vectors in noninvasive molecular imaging / Abhijit De ... [et al.] Retroviral modification of mesenchymal stem cells for gene therapy of hemophilia A / Christopher B. Doering. Transduction of murine hematopoietic stem cells and in vivo selection of gene-modified cells / Lucienne M. Ide, Elisabeth Javazon, and H. Trent Spencer. Assessment of CFTR function after gene transfer in vitro and in vivo / Uta Griesenbach ... [et al.] Oncolytic adenoviruses for cancer gene therapy / Ta-Chiang Liu, Stephen H. Thorne, and David H. Kirn. Design of trans-splicing adeno-associated viral vectors for Duchenne muscular dystrophy gene therapy / Yi Lai ... [et al.] Prevention of type 1 diabetes in NOD mice by genetic engineering of hematopoietic stem cells / Jessamyn Bagley, Charoui Tian, and John Iacomini. Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and John J. Rossi. Nanoparticle-mediated gene delivery to the lung / Rajagopal Ramesh. Retroviral-mediated gene therapy for the differentiation of primary cells into a mineralizing osteoblastic phenotype / Jennifer E. Phillips and Andrâes J. Garcâia. In vivo siRNA delivery to the mouse hypothalamus shows a role of the co-chaperone XAP2 in regulating TRH transcription / Stâephanie Decherf, Zahra Hassani, and Barbara A. Demeneix. Efficient retroviral gene transfer to epidermal stem cells / Pedro Lei and Stelios T. Andreadis -- v. 2. Design and characterization of gene transfer vectors. Purification of retrovirus particles using heparin affinity chromatography / Marâia de las Mercedes Segura, Amine Kamen, and Alain Garnier. Scaleable purification of adenovirus vectors / John O. Konz, Lee R. Pitts, and Sangeetha L. Sagar. Quantifying the titer and quality of adenovirus stocks / Fubao Wang ... [et al.] Chromatography-based purification of adeno-associated virus / Richard H. Smith, Linda Yang, and Robert M. Kotin. Spectroscopic methods for the physical characterization and formulation of nonviral gene delivery systems / Salvador F. Ausar, Sangeeta B. Joshi, and C. Russell Middaugh. Real-time multiple particle tacking of gene nanocarriers in complex biological environments / Samuel K. Lai and Justin Hanes. Production of lentiviruses displaying "early-acting" cytokines for selective gene transfer into hematopoietic stem cells / E. Verhoeyen, D. Náegre, and F.L. Cosset. Fiber-modified adenoviruses for targeted gene therapy / Hongju Wu and David T. Curiel. PEGylated adenovirus for targeted gene therapy / Catherine R. O'Riordan and Antonius Song. Transposon-based mutagenesis generates diverse adeno-associated viral libraries with novel gene delivery properties / James T. Koerber and David V. Schaffer. Photochemical enhancement of DNA delivery by EGF receptor targeted polyplexes / Anette Bonsted ... [et al.] Reducing the genotoxic potential of retroviral vectors / Ali Ramezani, Teresa S. Hawley, and Robert G. Hawley. Evaluation of promoters for use in tissue-specific gene delivery / Changyu Aheng and Bruce J. Baum. Adenovirus-mediated transduction of auto-and dual-regulated transgene expression in mammalian cells / Valeria Gonzalez-Nicolini and Martin Fussenegger. Regulated expressioin of adenoviral vectors-based gene therapies : therapeutic expression of toxins and immune-modulators / James F. Curtin ... [et al.] Liver-directed gene therapy using the sleeping beauty transposon system / Lalitha R. Belur, R. Scott McIvor, and Andrew Wilber. Generation and functional analysis of zinc finger nucleases / Toni Cathomen ... [et al.] Conditional gene expression and knockdown using lentivirus vectors encoding shRNA / Jolanta Szulc and Patrick Aebischer.