Bioconjugate Techniques.
Title:
Bioconjugate Techniques.
Author:
Hermanson, Greg T.
ISBN:
9780080527901
Personal Author:
Edition:
1st ed.
Physical Description:
1 online resource (813 pages)
Contents:
Front Cover -- Bioconjugate Techniques -- Copyright Page -- Detailed Contents -- Preface -- Acknowledgments -- Part I: Bioconjugate Chemistry -- Chapter 1. Functional Targets -- 1. Modification of Amino Acids, Peptides, and Proteins -- 2. Modification of Sugars, Polysaccharides, and Glycoconjugates -- 3. Modification of Nucleic Acids and Oligonucleotides -- 4. Creating Specific Functional Groups -- 5. Blocking Specific Functional Groups -- Chapter 2. The Chemistry of Reactive Groups -- 1. Amine-Reactive Chemical Reactions -- 2. Thiol-Reactive Chemical Reactions -- 3. Carboxylate-Reactive Chemical Reactions -- 4. Hydroxyl-Reactive Chemical Reactions -- 5. Aldehyde- and Ketone-Reactive Chemical Reactions -- 6. Active Hydrogen-Reactive Chemical Reactions -- 7. Photoreactive Chemical Reactions -- Part II: Bioconjugate Reagents -- Chapter 3. Zero-Length Cross-linkers -- 1. Carbodiimides -- 2. Woodward's Reagent K -- 3. N, N'-Carbonyldiimidazole -- 4. Schiff Base Formation and Reductive Amination -- Chapter 4. Homobifunctional Cross-linkers -- 1. Homobifunctional NHS Esters -- 2. Homobifunctional Imidoesters -- 3. Homobifunctional Sulfhydryl-Reactive Cross-linkers -- 4. Difluorobenzene Derivatives -- 5. Homobifunctional Photoreactive Cross-linkers -- 6. Homobifunctional Aldehydes -- 7. Bis-epoxides -- 8. Homobifuncrional Hydrazides -- 9. Bis-diazonium Derivatives -- 10. Bis-alkylhalides -- Chapter 5. Heterobifunctional Cross-linkers -- 1. Amine-Reactive and Sulfhydryl-Reactive Cross-linkers -- 2. Carbonyl-Reactive and Sulfhydryl-Reactive Cross-linkers -- 3. Amine-Reactive and Photoreactive Cross-linkers -- 4. Sulfhydryl-Reactive and Photoreactive Cross-linkers -- 5. Carbonyl-Reactive and Photoreactive Cross-linkers -- 6. Carboxylate-Reactive and Photoreactive Cross-linkers -- 7. Arginine-Reactive and Photoreactive Cross-linkers.
Chapter 6. Trifunctional Cross-linkers -- 1. 4-Azido-2-nitrophenylbiocytin-4-nitrophenyl ester -- 2. Sulfo-SBED -- Chapter 7. Cleavable Reagent Systems -- 1. Cleavage of Disulfides by Reduction -- 2. Periodate-Cleavable Glycols -- 3. Dithionite-Cleavable Diazo Bonds -- 4. Hydroxylamine-Cleavable Esters -- 5. Base Labile Sulfones -- Chapter 8. Tags and Probes -- 1. Fluorescent Labels -- 2. Bifunctional Chelating Agents and Radioimmunoconjugates -- 3. Biotinylation Reagents -- 4. lodination Reagents -- Part III: Bioconjugate Applications -- Chapter 9. Preparation of Hapten-Carrier Immunogen Conjugates -- 1. The Basis of Immunity -- 2. Types of Immunogen Carriers -- 3. Carbodiimide-Mediated Hapten-Carrier Conjugation -- 4. NHS Ester-Mediated Hapten-Carrier Conjugation -- 5. NHS Ester-Maleimide Heterobifunctional Cross-linker- Mediated Hapten-Carrier Conjugation -- 6. Active-Hydrogen-Mediated Hapten-Carrier Conjugation -- 7. Glutaraldehyde-Mediated Hapten-Carrier Conjugation -- 8. Reductive-Amination-Mediated Hapten-Carrier Conjugation -- Chapter 10. Antibody Modification and Conjugation -- 1. Preparation of Antibody-Enzyme Conjugates -- 2. Preparation of Labeled Antibodies -- Chapter 11. Immunotoxin Conjugation Techniques -- 1. Properties and Use of Immunotoxin Conjugates -- 2. Preparation of Immunotoxin Conjugates -- Chapter 12. Preparation of Liposome Conjugates and Derivatives -- 1. Properties and Use of Liposomes -- 2. Derivatization and Activation of Lipid Components -- 3. Use of Glycolipids and Lectins to Effect Specific Conjugations -- 4. Antigen or Hapten Conjugation to Liposomes -- 5. Preparation of Antibody-Liposome Conjugates -- 6. Preparation of Biotinylated or Avidin-Conjugated Liposomes -- 7. Conjugation of Proteins to Liposomes -- Chapter 13. Avidin-Biotin Systems -- 1. The Avidin-Biotin Interaction.
2. Use of Avidin-Biotin in Assay Systems -- 3. Preparation of Avidin or Streptavidin Conjugates -- 4. Preparation of Fluorescently Labeled Avidin or Streptavidin -- 5. Preparation of Hydrazide-Activated Avidin or Streptavidin -- 6. Biotinylation Techniques -- 7. Determination of the Level of Biotinylation -- Chapter 14. Preparation of Colloidal-Gold-Labeled Proteins -- 1. Properties and Use of Gold Conjugates -- 2. Preparation of Mono-disperse Gold Suspensions for Protein Labeling -- 3. Preparation of Protein A-Gold Complexes -- 4. Preparation of Antibody-Gold Complexes -- 5. Preparation of Lectin-Gold Complexes -- 6. Preparation of Avidin-Gold or Streptavidin-Gold Complexes -- Chapter 15. Modification with Synthetic Polymers -- 1. Protein Modification with Activated Polyethylene Glycols -- 2. Protein Modification with Activated Dextrans -- Chapter 16. Enzyme Modification and Conjugation -- 1. Properties of Common Enzymes -- 2. Preparation of Activated Enzymes for Conjugation -- 3. Preparation of Biotinylated Enzymes -- Chapter 17. Nucleic Acid and Oligonucleotide Modification and Conjugation -- 1. Enzymatic Labeling of DNA -- 2. Chemical Modification of Nucleic Acids and Oligonucleotides -- References -- Suppliers of Reagents and Devices for Bioconjugate Applications -- Index.
Abstract:
Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications
* Suggests sources for all key reagents.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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