Cover image for Experimental Glycoscience : Glycobiology.
Experimental Glycoscience : Glycobiology.
Title:
Experimental Glycoscience : Glycobiology.
Author:
Taniguchi, Naoyuki.
ISBN:
9784431779223
Personal Author:
Physical Description:
1 online resource (515 pages)
Contents:
Preliminary -- Part 1 / Glycosyltransferase Genes -- Convenient Synthesis of Glycan-Related Oligosaccharides and Their Transformation into Neoglycoconjugates Using Enzymatic Methods -- Comparison of Glycosyltransferase Families Using the Profile Hidden Markov Model -- N -Acetylglucosaminyltransferases Involved in N -Glycan Biosynthesis -- UDP- N -Acetyl-d -Galactosamine:polypeptide N -Acetylgalactosaminyltransferases (ppGaNTases, pp-GalNAc-Ts, EC 2.4.1.41) -- Glycosyltransferase Family with β4GT Motif (β4Gal-T and β4GalNAc-T Family) -- β1,3-glycosyltransferase Gene Family and IGnT Gene Family -- O -Mannosyltransferase and POMGnT1 -- Glycosyltransferases Involved in O-fucose Glycan Synthesis -- Fucosyltransferase (α1,2/ α1,3/ α1,4-fucosyltransferases) -- FUT8 Assay -- Sialyltransferases -- Glucuronyltransferases Involved in the HNK-1 Biosynthesis -- α1,4-Linkage Glycosyltransferases -- Glycosyltransferases Involved in the Biosynthesis of Glycolipids -- Sialyltransferases and Other Enzymes Involved in the Biosynthesis of Gangliosides -- Heparan Sulfate Synthases and Related Genes -- Chondroitin Sulfate Biosynthesis and Related Genes -- Biosynthesis of Keratan Sulfate -- Hyaluronan Synthase Assay -- Glycosylphosphatidylinositol-Anchored Protein Biosynthesis and Related Genes in Mammalian Cells -- Cloning of Genes for N-linked Oligosaccharide Synthesis in Endoplasmic Reticulum -- N -acetylglucosamine-6- O -sulfotransferases -- Sulfotransferases Involved in Sulfation of Glycosaminoglycans -- Sulfatide Synthase -- CMP- N -acetylneuraminic Acid Hydroxylase -- Nucleotide Sugar Transporter Genes and Their Functional Analysis -- Processing Enzymes Involved in N -glycan Biosynthesis and Related Genes: the Golgi N -glycan Processing α-mannosidase II and α-mannosidase IIx -- Sialidase Genes.

Release of Sugar Chains from Glycosphingolipids -- Heparan Sulfate Endosulfatase Assay -- Sphingolipid Activator Proteins -- Affinity Purification of Recombinant Galectins -- Siglec Family -- Part 2 / Functional Analyses of Sugar Chains -- NKT Cells and Their Recognition of Glycolipids -- Carbohydrate Ligands for Selectins in Immune Cell Trafficking -- Carbohydrate Recognition by Cytokines and Its Relevance to Their Physiological Activities -- Detection of Mouse MGL1/CD301a by a Specific Monoclonal Antibody -- Roles of Serum Lectins in Host Defense -- Siglec-2 Is a Key Molecule for Immune Response -- Galectin and Immune System: Toward Clinical Applications -- Novel Glycolipids in Developing Brain: Determination by Nano-LC/MS/MS -- Functional Roles of the HNK-1 Carbohydrate and Polysialic Acid in the Nervous System -- Neuronal Function of Sulfatide -- Accumulation of Alzheimer β-amyloid via Ganglioside Clusters -- Chondroitin Sulfate Proteoglycans and Neuronal Network Formation -- Processing of Glycosyltransferases by Alzheimer's β-secretase (BACE1) -- Chondroitin Sulfate Proteoglycans: A Key Substance for Central Nervous System Injury Repair -- A Cytoplasmic Peptide: N -Glycanase and ER-Associated Degradation -- Ubiquitination of Glycoproteins in the Cytosol -- Degradation of Misfolded Glycoproteins in the Endoplasmic Reticulum -- Retrograde Transport of Glycolipid-Bound Toxins -- Degradation of Hyaluronan and Its Disorder -- Recent Advances in Enzyme Replacement Therapy for Lysosomal Diseases -- Developing an Assay System for the Hemagglutinin Mutations Responsible for the Binding of Highly Pathogenic Avian Influenza A Viruses to Human-Type Receptors -- Helicobacter pylori Growth Assay for Glycans -- Binding Properties of Clostridium botulinum Type C Progenitor Toxin -- Fucosylation and Cancer.

Biological Significance of Mucins Produced by Epithelial Cancer Cells -- Regulation of Glycosyltransferases for O-Glycan Core Structure in Cancer and Their Relations to Metastasis -- Roles of Carbohydrate-Mediated Cell Adhesion in Cancer Progression -- Conditional Transgenic Mouse of Hyaluronan Synthase: A Potential Model of Advanced Breast Cancer -- Proteoglycans in Tissue Regeneration -- Stem Cell Glycobiology -- Human Embryonic Stem (ES) Cells and Induced Pluripotent Stem (iPS) Cells Are Defined by Carbohydrate Markers -- Glycoantigens in Xenotransplantation -- Glycoconjugates in Spermatogenesis -- Recognition Mechanism of Egg and Sperm Based on Sugar Chains -- Functional Analysis of Sugar Chains Using a Genome-Wide RNAi System in Drosophila -- Functional Glycomics at the Level of Single Cells: Studying Roles of Sugars in Cell Division, Differentiation, and Morphogenesis with 4D Microscopy -- Studies on Functions of Notch O -Fucosylation in Drosophila -- Membrane Microdomain as a Platform of Carbohydrate-Mediated Interactions During Early Development of Medaka Fish -- Functions of Heparan Sulfate Proteoglycans in Morphogenesis -- Fukutin and Fukuyama Congenital Muscular Dystrophy -- Congenital Muscular Dystrophies Due to the Glycosylation Defect -- Molecular Diagnosis of Congenital Disorders of Glycosylation -- Research in Japan Has Contributed to the Understanding of GPI Anchor Deficiency -- Aberrant Expression of Sialidase in Cancer and Diabetes -- Insulin Resistance and Type 2 Diabetes as Microdomain Disease: Implication of Ganglioside GM3 -- Incompleteness of O -glycans at the Hinge Region of IgA1 and IgA Nephropathy -- β4-Galactosyltransferase Deficiency and IgA Nephropathy -- Glycosyltransferases Involved in the Biosynthesis of IgA O -Glycans -- Importance of Sugar Chains in the Function of Growth Factor Receptors.

Analysis of N -glycan of Growth Factor Receptors -- The Midkine Family and Its Receptors -- N -glycans Regulate Integrin α5β1 Functions -- Blood Group Antigens: Blood Group Carbohydrate Antigens -- Bombay, Lewis and Ii Blood Group Antigens on Human Erythrocytes -- Blood Cells in Glycobiology -- Part 3 / Glycosyltransferase Gene KO Mice -- Fucosyltransferase-9 Knockout Mouse -- Knockout Mice of α1,6 Fucosyltransferase (Fut 8) -- Glucuronyltransferase (GlcAT-P) Gene-Deficient Mice -- KO Mice of β1,4-galactosyltransferase-I -- Sulfotransferases -- KO Mice of Cerebroside Sulfotransferase -- KO Mice of β1,4- N -acetylgalactosaminyltransferase (GM2/GD2 Synthase) -- KO Mice of α-2,8-sialyltransferase (GD3 Synthase) -- Double KO Mice of β-1,4- N -acetylgalactosaminyltransferase (GM2/GD2 Synthase) and α-2,8-sialyltransferase (GD3 Synthase) -- Part 4 / Infrastructures and Research Resources -- Cell Lines for Glycobiology -- Sugar Chain-Related Genes: Glycosyltransferases Expression System -- GALAXY Database and Pyridylaminated Oligosaccharide Library -- Mass Production of Sugar Intermediates for the Synthesis of Functional Oligosaccharides -- A Database System for Glycogenes (GGDB) -- CabosDB: Carbohydrate Sequencing Database -- GlycoEpitope: A Database of Carbohydrate Epitopes and Antibodies -- A Novel Lectin-Affinity Database for Structural Glycomics -- Glycoconjugate Data Bank -- KEGG GLYCAN for Integrated Analysis of Pathways, Genes, and Structures -- DNA Microarray in Glycobiology -- Lectin Microarray -- Carbohydrate Microarray for Deciphering the Information Embedded in Oligosaccharide Structures -- Back matter.
Abstract:
This book aims to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry. The protocols are all self-contained descriptions of the equipment and reagents needed, followed by details of the experimental procedure.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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