Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
Title:
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
Author:
Abdelhamid, Mahmoud A. S.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (432 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62378