Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
Title:
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
Author:
Ahmad, Misbha Ud Din
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (386 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62469