Rapid Isolation of the Mitoribosome from HEK Cells
Title:
Rapid Isolation of the Mitoribosome from HEK Cells
Author:
Aibara, Shintaro
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (573 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57877