Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Title:
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Author:
Ariyaratne, Menaka
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (796 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60009