Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Title:

Author:

Ash, Philip A.

Personal Author:

Ash, Philip A.

Publication Information:

Cambridge, MA MyJoVE Corp 2016

Physical Description:

online resource (601 seconds)

Series:

Biochemistry

General Note:

Title from resource description page

Abstract:

Here, we describe a technique, protein film infrared electrochemistry, which allows immobilized redox proteins to be studied spectroscopically under direct electrochemical control at a carbon electrode. Infrared spectra of a single protein sample can be recorded at a range of applied potentials and under a variety of solution conditions.

Reading Level:

For undergraduate, graduate, and professional students

Subject Term:

Biochemistry

Electronic Access:

https://www.jove.com/t/55858

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