Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Title:
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Author:
Asher, Anthony
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (745 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58393