Using High Content Imaging to Quantify Target Engagement in Adherent Cells
Title:
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
Author:
Axelsson, Hanna
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (443 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58670