Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Title:
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Author:
B. Kumar, Ramakrishnan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (649 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55148