Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
Title:
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
Author:
Barczewski, Adam H.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (782 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We describe protocols for the structure determination of the IKK-binding domain of NEMO by X-ray crystallography. The methods include protein expression, purification and characterization as well as strategies for successful crystal optimization and structure determination of the protein in its unbound form.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60339