Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Title:
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Author:
Becke, Tanja D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (673 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58167