Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Title:
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Author:
Bezstarosti, Karel
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (714 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59079