Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Title:
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Author:
Brennich, Martha E.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (669 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54861