Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
Title:
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
Author:
Cabral, Fatima
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (534 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56993