A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
Title:
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
Author:
Cai, Xiongwei
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (525 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample buffer.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56855