Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Title:
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Author:
Carija, Anita
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (664 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57470