Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
Title:
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
Author:
Desai, Malhar
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (438 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Interactions of transcription factors (TFs) with the RNA polymerase are usually studied using pulldown assays. We apply a Biolayer Interferometry (BLI) technology to characterize the interaction of GrgA with the chlamydial RNA polymerase. Compared to pulldown assays, BLI detects real-time association and dissociation, offers higher sensitivity, and is highly quantitative.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59687