Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Title:
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Author:
Dowdle, Megan E.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (396 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56031