Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
Title:
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
Author:
Eble, Johannes A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (758 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57334