Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Title:
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Author:
Elajaili, Hanan B.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (405 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58461