Laboratory Scale Production and Purification of a Therapeutic Antibody
Title:
Laboratory Scale Production and Purification of a Therapeutic Antibody
Author:
Elgundi, Zehra
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (594 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55153