Considerations for Routine Imaging -- Entering the Portal: Understanding the Digital Image Recorded Through a Microscope -- Quantitative Biological Image Analysis -- The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics -- Design and Function of a Light-Microscopy Facility -- Advanced Methods and Concepts -- Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls -- Quantitative FRET Microscopy of Live Cells -- Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells -- Single Fluorescent Molecule Tracking in Live Cells -- From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins -- Cutting-Edge Applications and Utilities -- Towards Imaging the Dynamics of Protein Signalling -- New Technologies for Imaging and Analysis of Individual Microbial Cells -- Imaging Parasites in Vivo -- Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells -- High-Throughput/High-Content Automated Image Acquisition and Analysis -- Cognition Network Technology – A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents -- High-Content Phenotypic Cell-Based Assays.