Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
Title:
Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
Author:
Banda, Omar A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (490 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60383