Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
Title:
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
Author:
Benny, Paula
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (554 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53642