Glutamine Flux Imaging Using Genetically Encoded Sensors
Title:
Glutamine Flux Imaging Using Genetically Encoded Sensors
Author:
Besnard, Julien
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (623 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51657