High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
Title:
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
Author:
Butterworth, Elizabeth
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (594 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56859