Simple, Affordable, and Modular Patterning of Cells using DNA
Title:
Simple, Affordable, and Modular Patterning of Cells using DNA
Author:
Cabral, Katelyn A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (539 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61937