Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
Title:
Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
Author:
Chen, Kevin Y.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (481 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61154