Permeabilization of Adhered Cells Using an Inert Gas Jet
Title:
Permeabilization of Adhered Cells Using an Inert Gas Jet
Author:
Cooper, Scott
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (501 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50612