2D and 3D Matrices to Study Linear Invadosome Formation and Activity
Title:
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
Author:
Di Martino, Julie
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (745 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
This protocol describes how to prepare a 2D mixed matrix, consisting of gelatin and collagen I, and a 3D collagen I plug to study linear invadosomes. These protocols allow for the study of linear invadosome formation, matrix degradation activity, and the invasion capabilities of primary cells and cancer cell lines.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54911