Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Title:
Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Author:
Abrams, Jennifer L.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (832 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This article describes the use of a firefly luciferase-GFP fusion protein to investigate in vivo protein folding in Saccharomyces cerevisiae. Using this reagent, refolding of a model heat-denatured protein can be monitored simultaneously by fluorescence microscopy and an enzymatic assay to probe the roles of proteostasis network components in protein quality control.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50432