Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Title:
Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Author:
Aigrain, Louise
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (927 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52208