Single-cell Microinjection for Cell Communication Analysis
Title:
Single-cell Microinjection for Cell Communication Analysis
Author:
Alberto, Anael Viana Pinto
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (599 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We describe here how to perform a single-cell microinjection of Lucifer Yellow to visualize cellular communication via gap-junctions in living cells, and provide some useful tips. We expect that this paper will help everyone to evaluate the degree of cellular coupling due to functional gap junctions. Everything described here could be, in principle, adapted to other fluorescent dyes with molecular weight below 1,000 Daltons.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50836