Generation of Myospheres From hESCs by Epigenetic Reprogramming
Title:
Generation of Myospheres From hESCs by Epigenetic Reprogramming
Author:
Albini, Sonia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (572 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51243