The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
Title:
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
Author:
Anderson, Mark C.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (408 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58367