A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
Title:
A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
Author:
Andersson-Rolf, Amanda
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (558 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol explains primary Lgr5-positve organoid culture and the subsequent performance of retroviral transduction. This enables Cre-inducible overexpression or knockdown of the delivered transgene and allows functional studies to be carried out in the novel in vitro organotypic model system.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51765