Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Title:
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Author:
Appelhans, Timo
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (666 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57690