Expression and Purification of Virus-like Particles for Vaccination
Title:
Expression and Purification of Virus-like Particles for Vaccination
Author:
Arevalo, Maria T.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (377 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54041