Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Title:
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Author:
Artemenko, Yulia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (640 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56411