Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Title:
Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Author:
Avezov, Edward
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (617 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We describe a method for analysis of the alteration of N-linked glycans through the early life of glycoproteins after their biosynthesis in mammalian cells. This is achieved by pulse-chase analysis of metabolically labeled glycans, enzymatic release from glycoproteins and examination by HPLC.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/1899