A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Title:
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Author:
Aydin, Halil
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (429 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
In the post-human genomics era, the availability of recombinant proteins in native conformations is crucial to structural, functional and therapeutic research and development. Here, we describe a test- and large-scale protein expression system in human embryonic kidney 293T cells that can be used to produce a variety of recombinant proteins.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/4041