Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
Title:
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
Author:
Bader, Verian
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (873 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/4132